�z��Û��΢�^�о��M(j��n)չ

��r(sh��)�g��2013-01-05 10:27 ��Դ�� : ��x��2238

ժҪ��Û��һ��(g��)��y�},��M�ܸ��ƌW(xu��)�҂��Û��}��˴��о��s��߀�]��һ�N�^��ص׵Ľ�Q��,��ڴ��ȱ��,��ˎ��ֻ�m��R�r(sh��)��(y��ng)��ʹ�á,��Ûͨ�÷��ȱ��,��ʹ�о��_ʼ�P(gu��n)ע�z��΢�^�о��Ŀǰ,��о��z��Û�ğ��c(di��n)֮һ��b�e��Û��ض��z��Ȼ��(y��ng)��PCR�U(ku��)��g(sh��),��ԭλ�s��g(sh��)�Լ�΢��Ԅ��侀��ȼ��g(sh��)�о��@Щ�ض��z��B(t��i)��,��ԇ�D�ҵ��Ա��@Щ�ض��z��Û�ķ��,��ȡһ��Ĵ�ʩ��@Щ�z��^��L��

�P(gu��n)�I�~��z��,��z��Û,��z��b��

�ڹ��,��˂��Ѿ��w��(x��)��B��γ� �z��΢��y(t��ng)�Q��z��,��z�(x��)��,��ž� ��,��z��ͽz���,��z�(x��)��û�� ෨̎��ˮ�Ĺ�ˇ��ռ��Ҫ��λ��ܶ�z�� (x��)��܉��|(zh��)��ɢ�ھ��w��,�,��ɰ��x��(x��)��ˮ�еđҸ��w��ճ�B��.�γ� ��w��z�(x��)��͘�(g��u)��˻��Ĺ� ��,��γ��Ҫ��,��ˣ��ˮ��̎��нz�(x��)��ǲ��ȱ�ٵ�,��еĽz��L��ֳ.�t��܌�(d��o)�½z��Û(��º��Q��Û),��ʹ�� ׃���ˮ�r(sh��)��ʧ,��ʹ�ó�ˮˮ�|(zh��) ��,��(y��n)��Ӱ��ˮ̎��S��\(y��n)��.��(y��n)�ؕr(sh��)�� ش�pʧ��ʮ��.��о��ߌ��M(j��n)�� ˴��о�,��l(f��)�F(xi��n)��Û�cˮ�|(zh��)��\(y��n)�Зl�� P(gu��n),��_��x��,��,��SBR��,��(qi��ng)��ؚ�ؿ��Ʒ�,��Ͽ��Û�� AB�ɼ��ؚ�ϵ�y(t��ng),��๤ˇ�ȷ��Ч��Û,��M��ˣ��چ��}�ď�(f��)�s��,�� 緶��(n��i),��Û�ԕr(sh��)��l(f��)��,��]��һ�N �^��ص׵ķ��η��,��һ��l(f��)��Û��Ͷ ��ķ��һ��ܺܿ콵��SVI ֵ,��@�ɷN��ǻ��Û ��ԭ��ᘌ��ض��NĽz��M(j��n)�п��Ƶķ� ��,��Բ��܏ĸ��Ͽ��ƽz��^�ȷ�ֳ.һ ��ֹͣ��ˎ��Û�F(xi��n)��п��ٴΰl(f��)��,�� ,��H��R�r(sh��)��(y��ng)��(y��ng)�á�

��Û��ض��ėl��½z��^�� ֳ��(d��o)��.��Դ_��z��Ԍ�� Û��A(y��)��Ϳ��һ��x��Ŀǰ,��b�e ��Û�Ľz��N�,��(y��ng)��PCR(poly— merase chain reaction.�ַQDNA��ø�?zh��n)��?y��ng).

��һ�NDNAƬ��w��ٔU(ku��)��ķ��)�U(ku��)�� g(sh��)��ԭλ�s��g(sh��)�Լ�΢��Ԅ��侀��ȼ� �g(sh��)�о��B(t��i)��,��(j��)��B(t��i)�� ȡһ��Ĵ�ʩ��@Щ�z��L.�Ա�� Û�İl(f��)��P(gu��n)�z��Û�о��ğ� �c(di��n)֮һ ,��

1 �z��ΑB(t��i)�c��B(t��i)��໥Ӱ�

J.Kappeler�� J(r��n)��z��Ĵ��L�c�� w��(n��i)��|(zh��)�U(ku��)ɢ��P(gu��n)��w��(n��i)�� |(zh��)��ȳ��ݶȷֲ�,��z��ͨ��һ�˻��߃� ��ߝ�Ȼ��|(zh��)�ķ��L,��˷N�ΑB(t��i)ʹ�z�� Ƶ��l��ȡ�ø��ĸ��(y��u)�ݣ�ʹ�NȺ�@�Ã�(y��u)��B(t��i)λ,��B(t��i)λռ��(y��u)�ݵ��r��,��z ��ջ��|(zh��)��˾��и��ߵ��L ��,��Ķ��A�ø��ĸ��(y��u)��,��

2��y(t��ng)��z��b��

1975��D.H.Eikelboom�� ״Ό�� еĽz��M(j��n)��b��.��x��26��(g��)��͵� �z��1981�ꌢ��U(ku��)��29��(g��)��,��@�N ��y(t��ng)��b��Ǹ��(j��)�z��ΑB(t��i)��Ⱦɫ ��(y��ng)��_��z��N��,��M��@�N��ã��Ǵ��һ��,��,��S.natans�� 1701,��0092,��0961��ͽz��ΑB(t��i)�S�l��׃��׃��@�N��ˆTҪ��^��,��茣�T��Ӗ(x��n),��t��׳��F(xi��n)�Д��e(cu��)�`��r��

3 ��W(xu��)��b��z��

��W(xu��)��һ�N�Է��΢��DNA�� RNA��A(ch��)�ķ��,��,��S��PCR��y

Ԕ��(x��)��(n��i)��Ո�鿴��֣�

����D(zhu��n)�d��ǳ��ڂ��f��Ϣ֮Ŀ��,��Ё�Դ��(bi��o)ע�e(cu��)�`��ַ��ĺϷ��(qu��n)��,��Ո��߳֙�(qu��n)��C��c��W(w��ng)(li��n)ϵ��҂��r(sh��)��,��h��,��x�x��